Сломали автономную публикацию с параметром ReadyToRun.
Результат:

System.IO.FileLoadException: Could not load file or assembly 'Microsoft.EntityFrameworkCore, Version=3.1.9.0, Culture=neutral, PublicKeyToken=adb9793829ddae60'. The located assembly's manifest definition does not match the assembly reference. (0x80131040)
File name: 'Microsoft.EntityFrameworkCore, Version=3.1.9.0, Culture=neutral, PublicKeyToken=adb9793829ddae60'